Journal: Clinical and Experimental Gastroenterology

Article Title: Quantification of Intestinal Lactobacillus Species in Children with Functional Constipation by Quantitative Real-Time PCR

doi: 10.2147/CEG.S250755

Figure Lengend Snippet: Specificity of the L. Casei Primer Pair Used in This Study

Article Snippet: Seven Lactobacillus reference strains ( L. casei ATCC 39392, L. paracasei ATCC 25598, L. rhamnosus ATCC 7469, L. plantarum ATCC 8014, L. reuteri ATCC 23272, L. fermentum ATCC 9338, L. acidophilus ATCC 4356) used to validate the assays in the study were ordered from the Iranian Research Organization for Science and Technology (IROST, Iran).

Techniques: Real-time Polymerase Chain Reaction

Journal: Clinical and Experimental Gastroenterology

Article Title: Quantification of Intestinal Lactobacillus Species in Children with Functional Constipation by Quantitative Real-Time PCR

doi: 10.2147/CEG.S250755

Figure Lengend Snippet: Prevalence of Lactobacillus Species, Detected by Quantitative PCR

Article Snippet: Seven Lactobacillus reference strains ( L. casei ATCC 39392, L. paracasei ATCC 25598, L. rhamnosus ATCC 7469, L. plantarum ATCC 8014, L. reuteri ATCC 23272, L. fermentum ATCC 9338, L. acidophilus ATCC 4356) used to validate the assays in the study were ordered from the Iranian Research Organization for Science and Technology (IROST, Iran).

Techniques:

Journal: Clinical and Experimental Gastroenterology

Article Title: Quantification of Intestinal Lactobacillus Species in Children with Functional Constipation by Quantitative Real-Time PCR

doi: 10.2147/CEG.S250755

Figure Lengend Snippet: Quantity of seven Lactobacillus species in the feces of constipated children and healthy controls. Statistical significance of observed differences in the amount of Lactobacillus species between both constipated and healthy groups was measured by the Mann–Whitney U -test. Bars represent standard errors. P < 0.05 was marked with one asterisk (∗), and P < 0.2 with two asterisks (∗∗). One-way ANOVA testing shows the significant differences in quantity (log 10 CFU/gram) between Lactobacillus species.

Article Snippet: Seven Lactobacillus reference strains ( L. casei ATCC 39392, L. paracasei ATCC 25598, L. rhamnosus ATCC 7469, L. plantarum ATCC 8014, L. reuteri ATCC 23272, L. fermentum ATCC 9338, L. acidophilus ATCC 4356) used to validate the assays in the study were ordered from the Iranian Research Organization for Science and Technology (IROST, Iran).

Techniques: MANN-WHITNEY

Journal:

Article Title: Cloning, Expression, and Characterization of Cadmium and Manganese Uptake Genes from Lactobacillus plantarum

doi:

Figure Lengend Snippet: Initial rates of cadmium uptake by E. coli and L. plantarum in APT medium a

Article Snippet: To identify MntA expression, crude cell extracts or total membrane proteins of two wild-type L. plantarum strains (ATCC 14917 and ATCC 8014) as well as two Mn 2+ -dependent mutants (mnd15-26 and mnd11-06) were prepared, separated by SDS-PAGE, and immunoblotted with an MntA antibody.

Techniques:

Journal:

Article Title: Cloning, Expression, and Characterization of Cadmium and Manganese Uptake Genes from Lactobacillus plantarum

doi:

Figure Lengend Snippet: Induction of the synthesis of mntA mRNA by Mn2+ starvation in L. plantarum ATCC 14917. Total RNA was isolated from L. plantarum ATCC 14917 Mn2+-starved (−) or Mn2+-sufficient (+) cells, treated with DNase I, and reverse transcribed with a specific antisense primer. The RT reaction mixture was used as a template to perform PCR to amplify a 1.1-kb fragment of mntA as described in the text. Each lane represents a separate experiment with four independent RNA preparations. MW, molecular weight.

Article Snippet: To identify MntA expression, crude cell extracts or total membrane proteins of two wild-type L. plantarum strains (ATCC 14917 and ATCC 8014) as well as two Mn 2+ -dependent mutants (mnd15-26 and mnd11-06) were prepared, separated by SDS-PAGE, and immunoblotted with an MntA antibody.

Techniques: Isolation, Reverse Transcription, Molecular Weight

Journal:

Article Title: Cloning, Expression, and Characterization of Cadmium and Manganese Uptake Genes from Lactobacillus plantarum

doi:

Figure Lengend Snippet: Identification of MntA in wild-type L. plantarum strains (A) and Mn2+-dependent mutants (B). Western blotting was done with an MntA antibody and protein extracts from L. plantarum strains and Cd2+ uptake mutants. (A) Lane 1, molecular mass standards (in kilodaltons); lanes 2 and 5, membrane protein from Mn2+-starved cells of ATCC 8014 and ATCC 14917, respectively; lanes 3 and 6, total protein from Mn2+-starved cells of ATCC 8014 and ATCC 14917, respectively; lanes 4 and 7, total protein from Mn2+-sufficient cells of ATCC 8014 and ATCC 14917, respectively. (B) Lanes 1 and 6, total protein from Mn2+-starved cells of ATCC 8014 and ATCC 14917, respectively; lanes 2 and 4, total protein from Mn2+-starved cells of mnd15-26 and mnd11-06, respectively; lanes 3 and 7, molecular weight standards; lane 5, membrane protein from Mn2+-starved cells of ATCC 8014.

Article Snippet: To identify MntA expression, crude cell extracts or total membrane proteins of two wild-type L. plantarum strains (ATCC 14917 and ATCC 8014) as well as two Mn 2+ -dependent mutants (mnd15-26 and mnd11-06) were prepared, separated by SDS-PAGE, and immunoblotted with an MntA antibody.

Techniques: Western Blot, Membrane, Molecular Weight

Journal: Pharmaceuticals

Article Title: Development and Characterization of Hydroxyethyl Cellulose-Based Gels Containing Lactobacilli Strains: Evaluation of Antimicrobial Effects in In Vitro and Ex Vivo Models

doi: 10.3390/ph16030468

Figure Lengend Snippet: Inhibitory activity of lactobacilli strains against pathogenic bacteria.

Article Snippet: For this, the antimicrobial actions of four lactobacilli strains ( Lacticaseibacillus rhamnosus ATCC 10863, Limosilactobacillus fermentum ATCC 23271, Lactiplantibacillus plantarum ATCC 8014 and Lactiplantibacillus plantarum LP-G18-A11) were screened against E. faecalis ATCC 29212, K. pneumoniae ATCC 700603, S. aureus ATCC 27853 and P. aeruginosa ATCC 2785.

Techniques: Activity Assay, Bacteria, Inhibition

Journal: Pharmaceuticals

Article Title: Development and Characterization of Hydroxyethyl Cellulose-Based Gels Containing Lactobacilli Strains: Evaluation of Antimicrobial Effects in In Vitro and Ex Vivo Models

doi: 10.3390/ph16030468

Figure Lengend Snippet: Antimicrobial activity of natrosol gel with lactobacilli strains.

Article Snippet: For this, the antimicrobial actions of four lactobacilli strains ( Lacticaseibacillus rhamnosus ATCC 10863, Limosilactobacillus fermentum ATCC 23271, Lactiplantibacillus plantarum ATCC 8014 and Lactiplantibacillus plantarum LP-G18-A11) were screened against E. faecalis ATCC 29212, K. pneumoniae ATCC 700603, S. aureus ATCC 27853 and P. aeruginosa ATCC 2785.

Techniques: Activity Assay, Inhibition